Specifications
Sample Material
- Crude cell lysates (10 000 - 50 000 cells)
- Extracted RNA (0.5 µg RNA)
Precision
- CV < 10%
Linear Dynamic Range
- 2.8 logs for a sample in a single well.
- Wider range upon sample dilution either prior to or after analysis
Probes
- Custom made 25-60 nt with fluorescence label for detection
- Oligo dT for target capture and immobilization
Beads
- High performance streptavidin coated magnetic beads
Sensitivity
-
0.5 attomoles of target transcript
Multiplex
- Up to 30-plex
Throughput
- 96 samples less control wells in a single assay (96 well form factor)
Protocol Time
- Total time for 96 samples stand TRAC 3-4 h, 1-2 h hands-on time
