Specifications

Sample Material

  • Crude cell lysates (10 000 - 50 000 cells)
  • Extracted RNA (0.5 µg RNA)

Precision

  • CV < 10%

Linear Dynamic Range

  • 2.8 logs for a sample in a single well. 
  • Wider range upon sample dilution either prior to or after analysis

Probes

  • Custom made 25-60 nt with fluorescence label for detection
  • Oligo dT for target capture and immobilization

Beads

  • High performance streptavidin coated magnetic beads

Sensitivity

  • 0.5 attomoles of target transcript

Multiplex

  • Up to 30-plex

Throughput

  • 96 samples less control wells in a single assay (96 well form factor)

Protocol Time

  • Total time for 96 samples stand TRAC 3-4 h, 1-2 h hands-on time